Mbd2^tm1Bh

Exon 2 was deleted and replaced with a promoter-less beta-geo cassette via homologous recombination. Northern blot confirmed the absence of normal transcript in homozygous mutant animals. RT-PCR analysis using primers to exon 1 and exon 3 detected a faint mutant read-through product. Western blot analysis of spleen and liver nuclear extracts from homozygous animals detected a small mutant protein presumed to be the translation product from exon 1, and would lack any functional domains. (J:68356)