Pafah1b1^tm1Or

Flanking loxP sites and a neo cassette were introduced to the first ATG codon via homologous recombination. The ATG and neo cassette were removed by mating mutant animals to a PGK-cre line. The deletion results in translation from the second methionine and produces a shorter protein of 345 amino acids (instead of 409 amino acids). The mutant allele was confirmed by Western blot analysis of brain extracts from heterozygous animals. No homozygous mutant animals were born. (J:69631)