Trpc4^tm1Bni

278 bp of the exon encoding transmembrane domains 4 and 5 and the adjacent downstream splice donor site were replaced with a PGK-neo cassette in the reverse transcriptional orientation. Northern blot and Western blot of brain homogenates and primary mouse vascular endothelial cells (MAECs) cultured from homozygous mutant animals confirmed the absence of gene expression. (J:68178)