Tg(LPV-TAg1135)11Tvd

A mutant SV40 T-antigen sequence deleted for amino acids 17-27 and the small t-specific splice donor site was used as the transgene. As a result the large T-antigen should be expressed, but not the small T-antigen. The mutant transgene was fused to the lymphotrophic papovavirus (LPV) enhancer and early promoter to target expression to B- and T-lymphocytes and choroid plexus epithelium. The mutant protein retains the ability to bind RB1, RBL1, and TRP53. Regulatory sequences direct expression of the transgene in B- and T-lymphocytes and choroid plexus epithelium. Western blot analysis of transgenic animals confirmed expression of the transgene in the thymus and spleen. (J:78681, J:89094)