The entire gene coding region was deleted and replaced with a lacZ-PGK-neo cassette via homologous recombination. Northern blot analysis of whole embryo total RNA showed an absence of gene expression in homozygous mutants. (J:60589)
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The entire gene coding region was deleted and replaced with a lacZ-PGK-neo cassette via homologous recombination. Northern blot analysis of whole embryo total RNA showed an absence of gene expression in homozygous mutants. (J:60589)