The floxed neo cassette was excised from Vegfatm3Pec by the in vivo expression of cre recombinase, leaving the isoform specific cDNA replacement intact. The resultant allele lacks exon 6 as well as splice sites and thereby produces a single isoform with intermediate solubility and receptor binding properties. The exclusive presence of this isoform was verified by quatitative RT-PCR. (J:75507)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count