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The gene was disrupted by replacement of exons 4-6 with a neomycin resistance cassette via homologous recombination. The targeted mutation results in the loss of a conserved DNA-binding motif called the Walker box, and also causes a frameshift. Homozygous mutant embryos were detected by PCR genotype analysis up to day E10.5. The targeted allele was confirmed by RT-PCR analysis of E8.5 embryos using primers flanking the deleted region. No RT-PCR products were detected. (J:61460)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
