5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette via homologous recombination. Absence of protein activity in the brain, lungs, and kidneys of homozygous mutant animals was confirmed by gelatin zymography. (J:42742)
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5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette via homologous recombination. Absence of protein activity in the brain, lungs, and kidneys of homozygous mutant animals was confirmed by gelatin zymography. (J:42742)