The gene was disrupted by replacement of part of exon 11 with a PGK-neo cassette via homologous recombination. Northern blot and RT-PCR analysis confirmed the absence of gene expression in homozygous mutant animals. (J:63253)
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The gene was disrupted by replacement of part of exon 11 with a PGK-neo cassette via homologous recombination. Northern blot and RT-PCR analysis confirmed the absence of gene expression in homozygous mutant animals. (J:63253)