Rxrg^tm1Ipc

A neomycin selection cassette replaced exons 3 and 4, which encode the DNA binding domain and are common to both isoforms generated from this locus. While normal transcript was undetected several types of aberrant transcript were detected in homozygous mutant mice by Northern blot and RT-PCR analyses, including 1.6 and 3.0 kb fragments derived from the exon 1b isoform and several others derived from the exon 1a isoform. The 1.6 kb fragment involves an aberrant splice between exon 1b and exon 5, resulting in an isolated ligand binding coding region. The 3.0 kb fragment is believed to be the result of cryptic splicing within the neo transgene or an intron. All of the aberrant transcripts lacked the DNA binding domain. (J:34890)