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The neo cassette inserted into intron 1 of Lhxtm3Bhr was excised by breeding with CMV-cre transgenic mice. The four missense mutations affecting conserved cysteine residues in exons 1 and 2, encoding the two LIM domains, were left intact. The conservation of the endogenous expression pattern was verified by whole mount in situ hybridization of homozygous mutant embryos. RT-PCR and sequence analyses showed normal expression and the exclusive presence of the missense mutations. Finally, immunocytochemical analysis confirmed the presence of protein in mutant mice. (J:63192)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
