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A construct containing rat Kcna1 sequence appended with a hemagglutinin tag (HA) and neo was incorporated at the enodogenous locus via homologous recombination. The rat sequence was inserted such that it would replace the Kcna5 coding sequence and be expressed by the Kcna5 promoter. Northern blot analysis confirmed the absence of Kcna5, while the presence of the rat Kcna1 was detected by RT-PCR analysis. HA epitope directed antibodies showed a 62 kDa protein in the heart tissue obtained from mutant mice, but not in brain tissue. (J:78122)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
