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A construct was generated in which a human EF-1alpha promoter was separated from a modified human SHC1 cDNA fused to an IRES-GFP by a floxed stop codon. The floxed stop codon allowed transcriptional control via the expression of cre recombinase. SHC1 codons encoding three tyrosine residues (positions 239, 240, 317) that are phosphorylated after cross linking were modified to encode phenylalanine. This creates a dominant-negative protein in certain signaling pathways. Breeding of mice carrying this allele with transgenic mice expressing cre resulted in the expression of the modified SHC1, though not GFP. (J:78180)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
