Snrpn^tm3Alb

A 6 kb region of the gene encompassing exon 2 was replaced with an HPRT-loxP-neo cassette via homologous recombination resulting in deletion of 32 amino acids from the smaller of 2 open reading frames. The larger downstream open reading frame remains intact. RT-PCR analysis of heterozygous mutant animals showed expression of only wild-type transcripts in mice carrying a maternally derived mutation, and in mice carrying a paternally derived mutation the mutant transcript and transcripts of the neo gene are expressed. The deletion does not affect maternal-paternal imprinting of the DNA. (J:56617)