Snrpn^tm2Alb

A 4.8 kb region of the gene encompassing exon 1 was replaced with a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Northern blot analysis using a probe complementary to downstream exons showed significantly reduced gene expression in animals carrying the mutation on a paternally derived chromosome, but not when the animals carried the mutation on a maternally derived chromosome. (J:70275)