Most of the coding region of the gene was replaced with an in frame lacZ floxed TK-neo cassette via homologous recombination. Southern blot and PCR genotype analysis confirmed the targeted allele in homozygous mutant animals. (J:72943)
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Most of the coding region of the gene was replaced with an in frame lacZ floxed TK-neo cassette via homologous recombination. Southern blot and PCR genotype analysis confirmed the targeted allele in homozygous mutant animals. (J:72943)