Kif3c^tm2Gsn

Two loxP sites were introduced to the intron and 5' untranslated region flanking exon 1, and a third loxP site was introduced with a PGK-TK/PGK-neo cassette directly downstream exon 1 via homologous recombination. The PGK-TK/PGK-neo cassette were removed by transient expression of cre recombinase in correctly targeted ES cells leaving the loxP sites flanking exon 1 intact. Gene expression in homozygous mutant animals appeared normal compared to wild-type animals as determined by Western blot analysis of brain lysates. (J:70607)