The entire coding region was replaced by a cassette containing IRES-tau-lacZ followed by a floxed HSV-TK and neo region. Transient transfection of ES cells resulted in the excision of the floxed selection sequence. (J:77642)
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The entire coding region was replaced by a cassette containing IRES-tau-lacZ followed by a floxed HSV-TK and neo region. Transient transfection of ES cells resulted in the excision of the floxed selection sequence. (J:77642)