Part of exon 5, exon 6, exon 7, and part of exon 8, were replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot and RT-PCR analysis. (J:39015)
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Part of exon 5, exon 6, exon 7, and part of exon 8, were replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot and RT-PCR analysis. (J:39015)