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Animals heterozygous for Tbptm1Ees were crossed to a cre-expressing line to facilitate excision of the neomycin resistance cassette. The resulting chimeric protein is composed of amino acids 1-24 followed by 2 tandem repeats of the FLAG tag and the entire C-terminus (amino acids 136-316). RNase protection assay and Western blot analysis confirmed that gene expression and steady-state accumulation of the protein in homozygous mutant animals is similar to that of the wild-type protein. (J:77714)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
