Aire^tm1Pltn

A neomycin resistance cassette replaced a genomic fragment containing part of exon 6. This mutation mimics a common human mutation of a termination codon in exon 6. RT-PCR analysis demonstrated that aberrant trasncripts were expressed, and immunohistochemisty experiments on sections of thymus liver and brain of homozygous mice confirmed that no detectable protein was made from this allele. (J:77873)