Elane^tm1Sds

The insertion of a neomycin selection cassette replaced exon 2, which encodes the histidine residue of the catalytic triad. Northern blot analysis of total RNA obtained from bone marrow myeloid cells showed that transcript encoded by the targeted allele was absent. Reprobing of the blot showed that the expression of the closely linked proteinase 3 was unaffected by the targeting event. An absence of elastocytic activity derived from the targeted locus was determined via kappa-elastin zymography. (J:47420)