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Exon 2 was replaced with a PGK-neo cassette via homologous recombination. Homozygous mutant embryos were observed at a frequency of 10% and 6% at E8.5 and E9.5, respectively, by PCR genotype analysis. Because homozygous mutant animals could not be obtained, embryonic stem cell lines from E3.5 homozygous mutant embryos were established. RT-CR and Western blot analysis of the mutant stem cell lines confirmed the absence of gene expression. (J:76298)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
