S1pr3^tm1Jch

Sequence encompassing the entire open reading frame, which is contained in the 5' region of exon 2, was flanked by an upstream loxP site in intron 1 and a downstream floxed neo cassette. The entire open reading frame and the neo transgene were excised via cre recombinase mediated deletion. Northern blot analysis and in situ hybridization showed an absence of transcript in homozygous mutant mice. A complete absence of encoded protein was determined via Western blot analysis using antibodies for the amino and carboxy termini. (J:71372)