Gphn^tm1Jrs

A neomycin selection cassette replaced exon 1 and upstream regulatory sequence. Northern blot analysis of total RNA obtained from brain tissue showed an absence of transcript in homozygous mutant mice. A lack of encoded protein was determined via Western blot analysis of protein extracts from the brain. Protein was undetected by staining of spinal cord sections from homozygous mutant mice. (J:77775)