A construct containing a downstream neomycin resistance gene and Mesp1 cDNA containing the entire coding region positioned at the start site of the Mesp2 gene was inserted into the Mesp2 locus. A non-coding region of Mesp2 was left intact and utilized for RT-PCR analysis, which showed that transcript from the Mesp2 locus was absent, while transcript from both the endogenous and transgenic Mesp1 loci were present. (J:49903)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6NCrlj x CBA/JNCrlj)F1
Targeted
Insertion
--
1
5
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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