Tg(CMV-Scnn1a)1352Rss

The transgene consists of a constituitive and ubiquitous cytomegalovirus promoter, an IgE intron, the rat Scnn1a (alpha ENaC; position -78 to +2149), and simian virus 40 large T antigen polyadenylation sequences. RT-PCR showed reconstitution of expression of the transgene in lung, kidney, skin, and colon tissue. Expression analysis of cardiac tissue showed that Scnn1a transcript is absent in wild-type mice and present in the transgenic mice. Transcript was undetected in liver tissue isolated from wild-type and transgenic mice. Reconstitution was additionally confirmed in lung tissue via in situ hybridization. (J:43583)