Del(19Poll-Dcpd)1Nmt

The gene was disrupted by replacement of exons 1-6 with a PGK-neo cassette via homologous recombination. The gene targeting event results in deletion of the translation initiation site, the BRCT motif, and half of the polymerase X domain of Poll. Homozygous mutant animals lack gene expression in testis as determined by Northern blot analysis. Analysis of the genomic region surrounding the Poll gene revealed an uncharacterized gene, named Dpcd, that is predicted to be transcribed from the opposite strand relative to Poll. The deletion of Poll also removes the first exon of Dpcd. (J:75779, J:97294)