Cel^tm1Dyh

Exon 4 was disrupted by the insertion of a neomycin resistance gene. The disruption occurred immediately upstream of the active site domains and precludes the processing of exon 11, which is essential for secretion of the protein from the pancreas. Western blot analysis of pancreatic homogenates from homozygous mutant mice showed an absence of protein product, truncated or otherwise. (J:32099)