A construct containing a beta-geo fusion marker as well as a splice-acceptor site and a picornaviral internal ribosome-entry site (IRES) was inserted upstream of exon 8 and 9. The construct was designed to prevent the translation of the functionally essential centromere targeting region. Southern blot analysis confirmed the targeting event. (J:45656)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count