A neomycin resistance gene replaced part of exon 3, exons 4-7, and part of exon 8 via homologous recombination. Homozygous mutant embryos were detected by PCR and Southern blot analysis. (J:76786)
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A neomycin resistance gene replaced part of exon 3, exons 4-7, and part of exon 8 via homologous recombination. Homozygous mutant embryos were detected by PCR and Southern blot analysis. (J:76786)