Limk2^tm1.1Tnak

This allele is a derivative of Limk2tm1Tnak. Cre-mediated recombination in vivo under control of the ACTB promoter excised the inserted neo cassette and exons 3, 4, 5, and 1t in the germline. Exons 3-5 encode the second LIM domain and part of the PDZ domain. Exon 1t encodes the initiation exon for the testis specific isoform of the protein. Western blot analysis did not detect protein in fibroblast cells derived from homozygous mutant embryos. Northern blot analysis of testis did not detect any mRNA in homozygous mutant mice. (J:73966)