Dlx5^tm1Levi

A targeting vector containg a promoterless lacZ gene and a floxed neomycin resistance gene under the control of a RSV promoter was used to delete a region spanning from within exon 1 to the 5' region of exon 3. RT-PCR analysis showed an absence of transcript in total RNA obtained from homozygous mutant embryos. This allele was used to generate a subsequent allele, Dlx5tm1.1Levi, which lacks the neomycin resistance gene. (J:56557)