Fer^tm1Pag

An aspartate to arginine mutation at position 743 (D743R) was introduced into exon 19 via homologous recombination in conjunction with a neo resistance cassette downstream of exon 20. The mutation is designed to inactivate the kinase domain by destabilizing the catalytic loop. Western blot analysis of homozygous mutant animals detected protein expression in testis but greatly reduced protein expression in liver. Kinase assays demonstrated complete absence of kinase activity in both testis and liver extracts from homozygous mutants. (J:67350)