Scn2a^tm1Mml

Most of exon 1, which encodes amino acids 1-89, was replaced with a neomycin resistance gene via homologous recombination. RNase protection assays using whole brain preparations from homozygous mutant animals revealed a greater than 85% reduction in gene expression compared to wild-type. Protein expression was barely detectable as determined by Western blot analysis. (J:76457)