Akt1^tm1Mbb

A neomycin resistance gene was used to replace exons 4 through 7 and the 5' portion of exon 8. Exon 5 is known to encode an lysine residue essential for catalytic activity. Expression was undetected in homozygous mutant animals by both Northern and Western blot analyses. The authors noted that the expression of Akt2 and Akt3 was normal in mice homozygous for the targeted allele. (J:72176)