Exon 2 was replaced with a splice acceptor (SA)-Gfp-PGK-neo cassette via homologous recombination. RT-PCR analysis verified the lack of gene expression in homozygous mutant animals. (J:76331)
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Exon 2 was replaced with a splice acceptor (SA)-Gfp-PGK-neo cassette via homologous recombination. RT-PCR analysis verified the lack of gene expression in homozygous mutant animals. (J:76331)