Ank3^tm1Bnt

A neomycin resistance cassette was inserted into exon 1b, which is an alternatively spliced exon found in transcripts only in brain. Northern blot analysis on RNA derived from homozygous brains demonstrated that no transcript containing exon 1b was expressed, while expression of transcripts containing other alternaltively spliced exons remained unaffected. Western blot analysis and immunohistochemistry experiments confirmed that little or no protein was seen in cerebellum. (J:51377)