The promoter and first exon of the gene were flanked with inserted loxP sites. In addition, an frt-flanked neomycin resistance cassette was inserted into first intron. RT-PCR analysis on RNA derived from homozygous embryos demonstrated that aberrant neo-fusion transcripts were produced from this allele in addition to reduced levels of normal mRNA. (J:74631)
Basic Information
(C57BL/6NCrlj x CBA/JNCrlj)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count