A single loxP site was inserted into intron 1 and a loxP-flanked neomycin selection cassette was inserted 3' to exon 3. The neomycin cassette was removed in ES cells by transient Cre expression prior to the production of chimeric mice. (J:67222)
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A single loxP site was inserted into intron 1 and a loxP-flanked neomycin selection cassette was inserted 3' to exon 3. The neomycin cassette was removed in ES cells by transient Cre expression prior to the production of chimeric mice. (J:67222)