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An HA tag followed by a stop codon was introduced into exon 8, and was followed by an IRES-lacZ gene and neomycin selection cassette that replaced exons 9, 10 and 11. Western blot analysis on homozygous embryos demonstrated that no detectable ENG protein was expressed from this allele; however, a beta-galactosidase protein was expressed under the control of the endogenous promoter. (J:73415)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
