Il11ra1^tm1Gos

A targeting event resulted in the insertion of a neomycin resistance gene into intron 8, followed by a truncated, promoterless Il11ra1 gene lacking exons 2-13. Northern blot experiments on RNA derived from kidney of homozygous mice demonstrated that an aberrant transcript was produced from this allele. It was a size consistent with the splicing around the neo cassette to produce a transcript that contains exon 1-8 fused to exons 2-13. Western blot analysis on kidney extracts confirmed that an aberrantly sized protein was expressed from this allele, although at lower levels than Il11ra1tm2Gos. (J:48920)