A floxed neomycin resistance cassette was inserted upstream of exon 3 and an additional loxP site was inserted downstream of the exon. Transient expression of Cre recombinase in ES cells excised both the neo cassette and exon 3. (J:75726)
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A floxed neomycin resistance cassette was inserted upstream of exon 3 and an additional loxP site was inserted downstream of the exon. Transient expression of Cre recombinase in ES cells excised both the neo cassette and exon 3. (J:75726)