Plin1^tm1Chan

An IRES-lacZ gene followed by a neomycin resistance cassette replaced a genomic fragment containing part of exon 2 and intron 2. Northern blot analysis demonstrated that the encoded transcript was not detectable in homozygous mice. Western blot anlaysis on extracts derived from adipocytes and testis of homozygous mice confirmed that no detectable protein is expressed from this allele. (J:66267)