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In frame insertion of a lacZ-PGK-neomycin resistance cassette into exon 1 disrupted the gene. Northern blot analyses of skeletal muscle using a 3' exon 1 cDNA probe and using an exon 4-9 cDNA probe did not detect transcript in homozygous mutant mice. Western blot analysis did not detect protein in limb muscle from homozygous mutant mice. Immunocytochemical studies of skeletal, smooth, and cardiac muscle did not detect protein in homozygous mutant mice. (J:32928)