A neomycin selection cassette was inserted into exon 4 at the sequence corresponding to codon 230. RT-PCR analysis on RNA derived from targeted ES cells demonstrated that no wild-type transcript was produced from this allele. (J:73414)
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A neomycin selection cassette was inserted into exon 4 at the sequence corresponding to codon 230. RT-PCR analysis on RNA derived from targeted ES cells demonstrated that no wild-type transcript was produced from this allele. (J:73414)