Bcl2l2^tm1Sco

A genomic fragment containing two-thirds of the coding region was replaced with a neomycin selection cassette flanked by loxP sites. The neomycin cassette was removed via Cre mediated recombination in the final allele. Northern blot analysis on testis RNA derived from homozygous mice demonstrated that no detectable transcript is produced from this allele, and western blot analysis confirmed that no detectable protein was present in brain, testis or pancreas of homozygous mice. (J:50338)