Zic2^tm1Jaru

A gene targeting event was designed to replace the first exon containing the start codon and encoding three of the five zinc fingers. However, unexpected recombination resulted in an allele with reduced expression. The allele retained exon 1 with the neomycin-resistance marker in the first intron, shown by Southern blot. This resulted in reduction (knockdown, kd) of Zic2 expression. (J:60644)