Psen1^tm1Bdes

Insertion of a neomycin cassette resulted in interruption of the open reading frame in exon 7 of the gene. This position corresponds to 10 amino acid residues carboxyterminal of the third transmembrane domain of the protein. Northern blot analysis of whole embryo RNA showed no detectable mRNA in homozygous mutant embryos. Western blot analysis showed an absence of the 17 kD C-terminal fragment of the protein in homozygous mutant embryos. (J:71037)