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A lacZ gene was inserted in-frame into the gene, fused to the endogenous transcription start site. A neomycin selection cassette followed the lacZ gene. Northern blot analysis on RNA derived from homozygous ES cells demonstrated that no detectable transcript was produced from this allele, and western blot analysis on homozygous ES cells confirmed that no stable protein is produced from this allele (data not shown). (J:60391)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
